Journal: The Journal of Neuroscience

Article Title: Chronic Morphine Treatment Impaired Hippocampal Long-Term Potentiation and Spatial Memory via Accumulation of Extracellular Adenosine Acting on Adenosine A 1 Receptors

doi: 10.1523/JNEUROSCI.0148-10.2010

Figure Lengend Snippet: Chronic morphine treatment attenuated the activity but did not decrease the expression of ENT2. A, Effect of different concentrations of NBTI, a selective inhibitor of ENTs, on the uptake of adenosine in the hippocampal synaptosomes prepared from saline- and chronic morphine-treated mice. Data are presented as the percentage of control and represent the mean ± SEM (n = 6). **p < 0.01 compared with vehicle group from saline-treated mice. *p < 0.05 compared with vehicle group from morphine-treated mice, one-way ANOVA with PLSD post hoc test. B, There is no significant alteration of expression of ENT1 and ENT2 in the hippocampus from chronic morphine mice. Data are represented as mean ± SEM (n = 5).

Article Snippet: After slightly washing, the membrane was incubated with anti-ENT1 goat polyclonal antibody (1:50 Santa Cruz Biotechnology, sc-48488), anti-ENT2 goat polyclonal antibody (1:50, Santa Cruz Biotechnology sc-48491), anti-PKC-α/β monoclonal antibody, anti-PKC-γ rabbit polyclonal antibody (1:10,000, Santa Cruz Biotechnology sc-211), anti-PKC-ε mouse monoclonal antibody(1:50, Santa Cruz Biotechnology sc-56944) in TBS plus 5% BSA and 0.1% Tween 20, followed by incubation with horseradish peroxidase-conjugated IgG (Calbiochem) as the secondary antibody.

Techniques: Activity Assay, Expressing, Saline, Control